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LabMedMolGe - Team

Annamaria Salvati

Annamaria Salvati

e-mail: asalvati@unisa.it

 

Laurea in Biologia cum laude, Università Degli Studi di Salerno (2012).

 

Dottorato in Scienze e Tecnologie Biomediche (2017). 

Borsista post-dottorato nell'ambito del progetto di ricerca dal titolo "Medicina personalizzata per strategie innovative in malattie neuro-psichiatriche e vascolari (PerMedNet) avente ad oggetto. "Analisi genomiche mediante tecnologie di sequenziamento massivo parallelo (NGS) di acidi nucleici (DNA e RNA) con tecnologie Illumina" presso il Dipartimento di Medicina, Chirurgia e Odontoiatria "SCUOLA MEDICA SALERNITANA", Università degli Studi di Salerno (2019 - 2020).

 

Borsista Biologa per l'implementazione della Rete Oncologica Campana in ambito aziendale presso AOU SS Giovanni di Dio e Ruggi d'Aragona (Ottobre 2020 - in corso). 

Competenze ed attività di ricerca:  Tecniche di Biologia cellulare e molecolare, Genomica e Proteomica. Analisi globale del ruolo dei microRNA nel carcinoma mammario.. Analisi del profilo di espressione genica mediante microarray e tecnologie di Sequenziamento Massivo Parallelo per l'analisi di espressione genica, esomi, trascrittomi, microRNA e modifiche epigenetiche. Utilizzo microscopia confocale e citofluorimetria a flusso.

Pubblicazioni:

  • Gigantino V, Salvati A, Giurato G, Palumbo D, Strianese O, Rizzo F, Tarallo R, Nyman TA, Weisz A, Nassa G. Identification of Antiestrogen-Bound Estrogen Receptor α Interactomes in Hormone-Responsive Human Breast Cancer Cell Nuclei. Proteomics. 2020 Oct;20(19-20):e2000135. doi: 10.1002/pmic.202000135. Epub 2020 Sep 16. PMID: 32865868.
  • Strianese O, Rizzo F, Ciccarelli M, Galasso G, D'Agostino Y, Salvati A, Del Giudice C, Tesorio P, Rusciano MR. Precision and Personalized Medicine: How Genomic Approach Improves the Management of Cardiovascular and Neurodegenerative Disease. Genes (Basel). 2020 Jul 6;11(7):747. doi: 10.3390/genes11070747. PMID: 32640513; PMCID: PMC7397223.
  • Salvati A, Gigantino V, Nassa G, Mirici Cappa V, Ventola GM, Cracas DGC, Mastrocinque R, Rizzo F, Tarallo R, Weisz A, Giurato G. Global View of Candidate Therapeutic Target Genes in Hormone-Responsive Breast Cancer. Int J Mol Sci. 2020 Jun 6;21(11):4068. doi: 10.3390/ijms21114068. PMID: 32517194; PMCID: PMC7312026.
  • Montico B, Colizzi F, Giurato G, Rizzo A, Salvati A, Baboci L, Benedetti D, Pivetta E, Covre A, Bo MD, Weisz A, Steffan A, Maio M, Sigalotti L, Fratta E. Loss of Spry1 reduces growth of BRAFV600-mutant cutaneous melanoma and improves response to targeted therapy. Cell Death Dis. 2020 May 22;11(5):392. doi: 10.1038/s41419-020-2585-y. PMID: 32444628; PMCID: PMC7244546.
  • Salvati A, Gigantino V, Nassa G, Giurato G, Alexandrova E, Rizzo F, Tarallo R, Weisz A. The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells. Cancers (Basel). 2019 Nov 4;11(11):1720. doi: 10.3390/cancers11111720. PMID: 31689915; PMCID: PMC6895927.
  • Nassa G, Giurato G, Salvati A, Gigantino V, Pecoraro G, Lamberti J, Rizzo F, Nyman TA, Tarallo R, Weisz A. The RNA-mediated estrogen receptor α interactome of hormone-dependent human breast cancer cell nuclei. Sci Data. 2019 Sep 16;6(1):173. doi: 10.1038/s41597-019-0179-2. PubMed PMID: 31527615; PubMed Central PMCID: PMC6746822.
  • Nassa G, Salvati A, Tarallo R, Gigantino V, Alexandrova E, Memoli D, Sellitto A, Rizzo F, Malanga D, Mirante T, Morelli E, Nees M, Åkerfelt M, Kangaspeska S, Nyman TA, Milanesi L, Giurato G, Weisz A. Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv. 2019 Feb 6;5(2):eaav5590. doi: 10.1126/sciadv.aav5590. eCollection 2019 Feb. PubMed PMID: 30775443; PubMed Central PMCID: PMC6365116
  • Rossi S, Cordella M, Tabolacci C, Nassa G, D'Arcangelo D, Senatore C, Pagnotto P, Magliozzi R, Salvati A, Weisz A, Facchiano A, Facchiano F. TNF-alpha and metalloproteases as key players in melanoma cells aggressiveness. J Exp Clin Cancer Res. 2018 Dec 28;37(1):326. doi: 10.1186/s13046-018-0982-1. PubMed PMID: 30591049; PubMed Central PMCID: PMC6309098.
  • Lacunza E, Montanaro MA, Salvati A, Memoli D, Rizzo F, Henning MF, Quiroga IY, Guillou H, Abba MC, Gonzalez-Baro MDR, Weisz A, Pellon-Maison M. Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells. Oncotarget. 2018 Jun 15;9(46):28141-28154. doi: 10.18632/oncotarget.25582. eCollection 2018 Jun 15. PubMed PMID: 29963267; PubMed Central PMCID: PMC6021339.
  • Giurato G, Nassa G, Salvati A, Alexandrova E, Rizzo F, Nyman TA, Weisz A, Tarallo R. Quantitative mapping of RNA-mediated nuclear Estrogen Receptor β interactome in human breast cancer cells. Sci Data. 2018 Mar 6;5:180031. doi: 10.1038/sdata.2018.31. PubMed PMID: 29509190; PubMed Central PMCID: PMC5839158.
  • Nassa G, Giurato G, Cimmino G, Rizzo F, Ravo M, Salvati A, Nyman TA. Zhu Y, Vesterlund M, Lehtiö J, Golino P, Weisz A, Tarallo R. Splicing of platelet resident pre-mRNAs upon activation by physiological stimuli results in functionally relevant proteome modifications. Sci Rep. 2018 Jan 11;8(1):498. doi: 10.1038/s41598-017-18985-5. PubMed PMID: 29323256; PubMed Central PMCID: PMC5765118.
  • Tarallo R, Giurato G, Bruno G, Ravo M, Rizzo F, Salvati A, Ricciardi L, Marchese G, Cordella A, Rocco T, Gigantino V, Pierri B, Cimmino G, Milanesi L, Ambrosino C, Nyman TA, Nassa G, Weisz A. The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol.2017 Oct 6;18(1):189. doi: 10.1186/s13059-017-1321-0. PubMed PMID: 29017520.
  • D'Arcangelo D, Facchiano F, Nassa G, Stancato A, Antonini A, Rossi S, Senatore C, Cordella M, Tabolacci C, Salvati A, Tarallo R, Weisz A, Facchiano AM, Facchiano A. PDGFR-alpha inhibits melanoma growth via CXCL10/IP-10: a multi-omics approach. Oncotarget. 2016 Oct 13. doi: 10.18632/oncotarget.12629. PubMed PMID: 27764787.
  • Selleri C, Montuori N, Salvati A, Serio B, Pesapane A, Ricci P, Gorrasi A, Li Santi A, Hoyer-Hansen G, Ragno P. Involvement of urokinase receptor in the cross-talk between human hematopoietic stem cells and bone marrow microenvironment. Oncotarget. 2016 Aug 8. doi: 10.18632/oncotarget.11115. PubMed PMID: 27517491.

 

Riassunti pubblicati in atti di convegni nazionali/internazionali:
  • Gigantino V, Salvati A, Nassa G, Giurato G, Rizzo F, Mirici Cappa V, Memoli D, Weisz A, Tarallo R. Functional effect of DOT1L pharmacological inhibi-tion in ERα-positive Ovarian Cancer cells. 61th Annual Meeting of the Can-cer Society. 6-8 November 2019, Naples, Italy.
  • Gigantino V, Salvati A, Alexandrova E, Giurato G, Memoli D, Rizzo F, Sellitto A, Malanga D, Nyman TA, Tarallo R, Weisz A and Nassa G. Pharmacological inhibition of the histone methyltransferase DOT1L as a new epigenetic approach to therapy for endocrine resistant breast cancer. 1st INTERNA-TIONAL AND 32nd Annual Conference of Italian Association of Cell Cultu-res (AICC). 1-2 October 2019, Catanzaro, Italy.
  • Giurato G, Nassa G, Salvati A, Tarallo R, Rizzo F, Gigantino V, Mautone L, Nyman TA, Weisz A. "Analysis of estrogen-mediated signaling reveals a novel epigenetic target for endocrine therapy-resistant breast cancer treatment". Proceeding of 4th National Congress SIPMEL "Pathology and Laboratory Medicine 4.0"; 23-25 October 2018, Catania (Italy).
  • Salvati A, Giurato G, Lorenza M, Nyman TA, Nassa G, Weisz A. “Inhibition of the histone methyltransferases DOT1L deregulates hormone responsive breast cancer cells methylome”. Proceeding of EMBO workshop “From Epigenome towards Epitranscriptome in Cell Fate Choice” 14-17 Ottobre 2018, Capri (Italia).
  • Nassa G, Salvati A, Giurato G, Tarallo R, Rizzo F, Gigantino V, Mautone L, Nyman TA, Weisz A. The histone methyl transferase DOT1L is a novel epigenetic target for therapy of endocrine-therapy resistant breast cancer. Proceeding of 30th Pezcoller Foundation; 25-26 June 2018, Trento (Italy).
  • Memoli D, Salvati A, Rizzo F, Weisz A. Role of GPAT2 silencing in small RNA landscape in human MDA-MB-231 breast cancer cells. National Ph.D Meeting. 22-24 March 2018, Salerno, Italy.
  • Nassa G, Salvati A, Giurato G, Tarallo R, Ravo M, Rizzo F, Nyman TA, Weisz A. The histone methyl transferase DOT1L modulates estrogen- mediated signaling in breast cancer cells. Proceeding of Young Scientist meeting SIPMeT “Phatobiology ; from phatogenesis to translational medicine” University of Milan “La Statale”, 15- 16 September 2017, Milano (Italy)
  • Weisz A, Nassa G, Giurato G, Salvati A, Gigantino V, Nyman TA, Milanesi L, Tarallo R. A proteogenomics approach for identification of molecular determinants of the oncosuppressive actions of Estrogen Receptor beta in breast cancer. Proceeding of III International Symposium on Profiling - ISPROF 2017 Caparica, Lisbon (Portugal) 4-7 September 2017
  • Memoli D, Rinaldi A, Giurato G, Salvati A, Rizzo F, Weisz A. Analysis of miRNA and piRNA biogenesis in breast cancer cells. Proceeding of 14th Annual Meeting of the Bioinformatics Italian Society 5-8 July 2017, Cagliari (Italy)
  • Nassa G, Salvati A, Giurato G, Tarallo R, Ravo M, Rizzo F, Alexandrova E, Nyman TA, Weisz A. Involvement of Histone Methyl Transferase DOT1L on estrogen-mediated transcriptional regulation in breast cancer. Proceeding of EACR-AACR-SIC 2ndSpecial Conference 2017; 24-27 June 2017, Florence (Italy)
  • Tarallo R, Nassa G, Giurato G, Cimmino G, Bruno G, Rizzo F, Ricciardi L, Salvati A, Marchese G, Golino P, Weisz A. Platelet activation affects pre-mRNA maturation of group of Transcripts useful as markers of acute coronary syndromes. Proceeding of 3rd Joint Meeting of Pathology and Laboratory Medicine; 3-4 October 2016, Pescara (Italy)
  • D’Arcangelo D, Facchiano F, Nassa G, Stancato A, Antonini A, Rossi S, Senatore C, Tabolacci C, Salvati A, Tarallo R, Weisz A, Facchiano AM, Facchiano A. Melanoma growth is inhibited by PDGFR-alpha via CXCL10/IP-10: a multi-omics approach. Proceeding of 58th Annual Meeting of the Italian Cancer Society “Relovutionary Road”; Palazzo della Gran Guardia, 5-8 September 2016, Verona (Italy)
  • Salvati A, Nassa G, Tarallo R, Ravo M, Rizzo F, Nyman TA, Weisz A. Impact of the histone methyl transferase DOT1L on estrogen signaling in breast cancer Proceeding of National Ph.D. Meeting 2016; Hotel Salerno, 7-9 April 2016, Salerno (Italy)
  • Memoli D, Giurato G, Nassa G, Salvati A, Rizzo F, Weisz A. Integration of RBBs, smallRNA-seq and RNA-seq for biological interpretation. Proceeding of 13th Annual Meeting of the Bioinformatics Italian Society; University of Salerno, 15-17 June 2016 (Italy)
  • Giurato G, Tarallo R, Salvati A, Nassa G, Ravo M, Rizzo F, Weisz A. Identification of RNAs specifically associated to ERβ and AGO2 proteins in breast cancer cells. Proceeding of XII Annual Meeting of Italian Bioinformatics Society; 2015 Milano, (Italy).
  • Bruno G, Tarallo R, Nassa G, Cimmino G, Giurato G, De Filippo MR, Ravo M, Rizzo F, Salvati A, Conte S, Pellegrino G, Cirillo P, Calabrò P, Öhman T, Nyman TA, Golino P, Weisz A. In vitro Platelets Activation Determines a Post-Transcriptional Proteome Reorganization Mediated by microRNAs. Proceeding of the 2nd Joint Meeting Congress of Pathology and Laboratory Diagnostics, 17-20 September 2014, Palermo, Italy. Am. J. Pathol. 184 (Suppl): pg. S21, Abs. HEM4. 2014
  • Salvati A, Nassa G, Giurato G, Bruno G, Ravo, Ricciardi L, Tammaccaro S, Carbone F, Tarallo R. Functional Interaction of Estrogen Receptor Beta and Argonaute 2 in Transcriptional Modulation of Gene Expression in Breast Cancer Cells. Atti delle Giornate della Facoltà di Farmacia e Medicina a Salerno, 22-23 May 2014. Fisciano, (Italy).
  • Bruno G, Nassa G, Cimmino G, Giurato G, De Filippo MR, Salvati A, Conte S, Pellegrino G, Cirillo P, Calabrò P, Öhman T, Nyman TA, Golino P, Tarallo R. microRNA-Mediated Post-Transcriptional Reorganization of the Platelet Proteome Induced by In Vitro Activation. Giornate di Facoltà di Farmacia e Medicina, University of Salerno, 22-23 May 2014; Fisciano (Italy)
  • Salvati A, Nassa G, Cimmino G, Giurato G, De Filippo MR, Ravo M, Rizzo F, Bruno G, Conte S, Pellegrino G, Cirillo P, Calabrò P, Tiina Öhman, Nyman TA, Weisz A, Paolo Golino P, Tarallo R. In Vitro Platelet Activation Affects microRNA Expression Profile Inducing Proteome Reorganization. Proceeding of the FEBS-IGB workshop 2014 “Decoding non-coding RNAs in development and cancer”; Hotel La Palma, 12-15 October 2014, Capri (Italy).
  • Giurato G, Nassa G, Tarallo R, Ravo M, Rizzo F, Stellato C, Bruno G, Salvati A, Ricciardi L, Cimmino G, Öhman T, Nyman TA, Golino P, Weisz A. Human Platelet Transcriptome Analysis Reveals Splicing Events Under Activating Stimuli. Proceeding of the 2nd Joint Meeting Congress of Pathology and Laboratory Diagnostics, 17-20 September 2014, Palermo, Italy. Am. J. Pathol. 184(Suppl): pg. S21, Abs. HEM3. 2014
  • Nassa G, Tarallo R, Bruno G, Giurato G, Hashim A, Ravo M, Rinaldi A, Rizzo F, Salvati A, Stellato C, Nyman TA, Weisz A. microRNA-mediated actions of unliganded estrogen receptor beta in breast cancer. Proceedings of the 2014 Annual Meeting at Experimental Biology, 26-30 April 2014. San Diego (USA). FASEB J 28(1):1138.1, 2014.
  • Tarallo R, Nassa G, Giurato G, Bruno G, Salvati A, Ricciardi L, Rocco T, Rizzo F, Weisz A. Estrogen Receptor Beta/Ago2 complex binds to breast cancer cell genome and mediates transcriptional gene regulation by the nuclear receptor. Proceedings of SIC55th SIC Annual Meeting WELCOME TO THE SOUTH “Understanding onc-OMICS for patient-tailored cancer therapy”. 23-26 September 2013, Catanzaro (Italy)
  • Selleri C, Montuori N, Rocco M, Serio B, Salvati A, Lisanti A, Gorrasi A, Ragno P. Soluble forms of uPAR regulate adhesion, proliferation and survival of KG1 leukemic cells. Proceedings of 44th Congresso Nazionale Società Italiana Ematologia (SIE); 20-23 October 2013, Verona (Italy).
  • Tarallo R, Nassa G, De Filippo MR, Giurato G, Ravo M, Rizzo F, Bruno G, Salvati A, Nyman TA, Weisz A. A nuclear complex comprising Argonaute 2 mediates transcriptional gene regulation by Estrogen Receptor Beta in human breast cancer cells. Proceedings of EMBO “Nuclear receptors: Linking molecules, genomes & physiology”. 6-10 September 2013, Sorrento (Italy).