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LabMedMolGe - Team

Giorgio Giurato

Giorgio Giurato

e-mail: ggiurato@unisa.it


Laurea in Bioinformatica cum laude, Alma Mater Studiorum, Università di Bologna (2009)

 

Dottorato in Fisiopatologia Sperimentale e Neuroscienze, Seconda Università degli Studi di Napoli (2012)

Socio Fondatore della start-up innovativa Genomix4Life S.r.l., spin-off dell'Università degli Studi di Salerno (www.genomix4life.com)

 

Competenze ed attività di ricerca: Sviluppo di metodi computazionali per l'analisi di dati da esperimenti di Next-Generation-Sequencing (RNA-Seq, ChIP-Seq, miRNA-Seq, RIP-Seq).  Analisi statistica di dati da microarray (profili di espressione di mRNA, microRNA e genotyping).

Pubblicazioni

  • Ferravante C, Memoli, D, Palumbo D, Ciaramella P, Di Loria A, D’Agostino Y, Nassa G, Rizzo F, Tarallo R, Weisz A. & Giurato G. HOME-BIO (sHOtgun MEtagenomic analysis of BIOlogical entities): a specific and exhaustive pipeline for handling metagenomic shotgun sequencing data. BMC Bioinformatics. 2020 In revision.
  • Saggese P, Sellitto A, Martinez, C, Giurato G, Nassa G, Rizzo F, Tarallo R & Scafoglio C. Metabolic regulation of epigenetic modifications and cell differentiation in cancer. Cancers. 2020 In revision.
  • Ricciardi L, Giurato G, Memoli D, Pietrafesa M, Dal Col J, Salvato I, Nigro A, Vatrella A, Caramori G, Casolaro V and Stellato C. Posttranscriptional gene regulatory networks in chronic airway inflammatory diseases: in silico mapping of RNA-binding protein expression in airway epithelium. Frontiers. ACCEPTED
  • Cannarella R, Salemi M, Condorelli RA, Cimino L, Giurato G, Marchese G, Cordella A, Romano C, La Vignera S, Calogero AE. SOX13 gene downregulation in peripheral blood mononuclear cells of patients with Klinefelter syndrome. Asian J Androl. 2020 Oct 27. doi: 10.4103/aja.aja_37_20. Epub ahead of print. PMID: 33109779.
  • Gigantino V, Salvati A, Giurato G, Palumbo D, Strianese O, Rizzo F, Tarallo R, Nyman TA, Weisz A, Nassa G. Identification of Antiestrogen-Bound Estrogen Receptor α Interactomes in Hormone-Responsive Human Breast Cancer Cell Nuclei. Proteomics. 2020 Oct;20(19-20):e2000135. doi: 10.1002/pmic.202000135. Epub 2020 Sep 16. PMID: 32865868.
  • Salvati A, Gigantino V, Nassa G, Mirici Cappa V, Ventola GM, Cracas DGC, Mastrocinque R, Rizzo F, Tarallo R, Weisz A, Giurato G. Global View of Candidate Therapeutic Target Genes in Hormone-Responsive Breast Cancer. Int J Mol Sci. 2020 Jun 6;21(11):4068. doi: 10.3390/ijms21114068. PMID: 32517194; PMCID: PMC7312026.
  • Sellitto A, D’Agostino Y, Alexadrova E, Lamberti J, Pecoraro G, Memoli D, Rocco D, Coviello E, Giurato G, Nassa G, Tarallo R, Weisz A, Rizzo F. Insights into the  role of Estrogen Receptor Beta in Triple Negative Breast Cancer. Cancers. 2020 Jun 5;12(6):1477. doi: 10.3390/cancers12061477.
  • Alexadrova E, Pecoraro G, Sellitto A, Melone V, Ferravante C, Rocco T, Guacci A, Giurato G, Nassa G, Rizzo F, Weisz A, Tarallo R. An overview of candidate therapeutic target genes in ovarian cancer. Cancers. 2020 Jun 4;12(6):1470. doi: 10.3390/cancers12061470.
  • Buccarelli M, Lulli V, Giuliani A, Signore M, Martini M, D'Alessandris QG, Giannetti S, Novelli A, Ilari R, Giurato G, Boe A, Castellani G, Spartano S, Marangi G, Biffoni M, Genuardi M, Pallini R, Marziali G, Ricci-Vitiani L. Deregulated expression of the imprinted DLK1-DIO3 region in Glioblastoma Stem-like Cells: tumor suppressor role of lncRNA MEG3. Neuro Oncol. 2020 May 27:noaa127. doi: 10.1093/neuonc/noaa127.PMID: 32459347.
  • Montico B, Colizzi F, Giurato G, Rizzo A, Salvati A, Baboci L, Benedetti D, Pivetta E, Covre A, Bo MD, Weisz A, Steffan A, Maio M, Sigalotti L, Fratta E. Loss of Spry1 reduces growth of BRAFV600-mutant cutaneous melanoma and improves response to targeted therapy. Cell Death Dis. 2020 May 22;11(5):392. doi: 10.1038/s41419-020-2585-y. PMID: 32444628; PMCID: PMC7244546.
  • Scalise M*, Torella M*, Marino F, Ravo M, Giurato G, Vicinanza C, Cianflone E, Mancuso T, Aquila I, Salerno L, Nassa G, Agosti V, De Angelis A, Urbanek K, Veltri P, Mastroroberto P, De Feo M, Viglietto G, Weisz A, Nadal-Ginard B#, Ellison GM#, Torella D. Atrial Myxomas Arise From Multipotent Cardiac Stem Cells. Eur Heart J. 2020 Apr 24:ehaa156. doi: 10.1093/eurheartj/ehaa156. Epub ahead of print. PMID: 32330934.
  • Spadaro, G., Giurato, G., Stellato, C., Marone, G. & Casolaro, V. Basophil degranulation in response to IgE ligation is controlled by a distinctive circadian clock in asthma. Allergy. 2020 Jan;75(1): 158-68
  • Alexandrova E, Giurato G, Saggese P, Pecoraro G, Lamberti J, Ravo M, Rizzo F, Rocco D, Tarallo R, Nyman TA, Collina F, Cantile M, Di Bonito M, Botti G, Nassa G, Weisz A. Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer. Mol Cell Proteomics. 2020 Feb;19(2):245-260. doi: 10.1074/mcp.RA119.001817. Epub 2019 Dec 2. PMID: 31792072; PMCID: PMC7000115.
  • Sellitto A, Geles K, D'Agostino Y, Conte M, Alexandrova E, Rocco D, Nassa G, Giurato G, Tarallo R, Weisz A, Rizzo F. Molecular and Functional Characterization of the Somatic PIWIL1/piRNA Pathway in Colorectal Cancer Cells. Cells. 2019 Nov 5;8(11):1390. doi: 10.3390/cells8111390. PMID: 31694219; PMCID: PMC6912267.
  • Salvati A, Gigantino V, Nassa G, Giurato G, Alexandrova E, Rizzo F, Tarallo R, Weisz A. The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells. Cancers (Basel). 2019 Nov 4;11(11):1720. doi: 10.3390/cancers11111720. PMID: 31689915; PMCID: PMC6895927.
  • Nassa G, Giurato G, Salvati A, Gigantino V, Pecoraro G, Lamberti J, Rizzo F, Nyman TA, Tarallo R, Weisz A. The RNA-mediated estrogen receptor α interactome of hormone-dependent human breast cancer cell nuclei. Sci Data. 2019 Sep 16;6(1):173. doi: 10.1038/s41597-019-0179-2. PubMed PMID: 31527615; PubMed Central PMCID: PMC6746822.
  • Vecchio, E., Golino, G., Pisano, A., Albano, F., Falcone, C., Ceglia, S., Iaccino, E,. Mimmi, S., Fiume, G., Giurato, G., Britti, D., Scala, G. & Quinto, I. IBTK contributes to B-cell lymphomagenesis in Eμ-myc transgenic mice conferring resistance to apoptosis. Cell Death Dis. 2019 Apr 11;10(4):320
  • Nassa G, Salvati A, Tarallo R, Gigantino V, Alexandrova E, Memoli D, Sellitto A, Rizzo F, Malanga D, Mirante T, Morelli E, Nees M, Åkerfelt M, Kangaspeska S, Nyman TA, Milanesi L, Giurato G, Weisz A. Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv. 2019 Feb 6;5(2):eaav5590. doi: 10.1126/sciadv.aav5590. eCollection 2019 Feb. PubMed PMID: 30775443; PubMed Central PMCID: PMC6365116
  • Salemi, M., Cannarella, R., Condorelli, R.A., Cimino, L., Ridolfo, F., Giurato, G., Romano, C., La Vignera, S. & Calogero A.E. Evidence for long noncoding RNA GAS5 up-regulationin patients with Klinefelter syndrome. BMC Med Genet. 2019 Jan 7;20(1):4.
  • Giurato G, Nassa G, Salvati A, Alexandrova E, Rizzo F, Nyman TA, Weisz A, Tarallo R. Quantitative mapping of RNA-mediated nuclear Estrogen Receptor β interactome in human breast cancer cells. Scientific Data. Vol. In Press. Pag.1-10 ISSN:2052-4463.
  • Guacci A, Cordella A, Rocco T, Giurato G, Nassa G, Rizzo F, Carlomagno C, Pepe S, Tarallo R, Weisz A. Identification of a novel truncating mutation in PALB2 gene by a multigene sequencing panel for mutational screening of breast cancer risk-associated and related genes. J Clin Lab Anal. 2018 Feb 27. doi: 10.1002/jcla.22418. [Epub ahead of print] PubMed PMID: 29484706.
  • Nassa G, Giurato G, Cimmino G, Rizzo F, Ravo M, Salvati A, Nyman TA. Zhu Y, Vesterlund M, Lehtiö J, Golino P, Weisz A, Tarallo R. Splicing of platelet resident pre-mRNAs upon activation by physiological stimuli results in functionally relevant proteome modifications. Sci Rep. 2018 Jan 11;8(1):498. doi: 10.1038/s41598-017-18985-5. PubMed PMID: 29323256; PubMed Central PMCID: PMC5765118.
  • Tarallo R, Giurato G*, Bruno G, Ravo M, Rizzo F, Salvati A, Ricciardi L, Marchese G, Cordella A, Rocco T, Gigantino V, Pierri B, Cimmino G, Milanesi L, Ambrosino C, Nyman TA, Nassa G, Weisz A. The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol. 2017 Oct 6;18(1):189. doi: 10.1186/s13059-017-1321-0. PubMed PMID: 29017520. *Co-first author.
  • Pistore C, Giannoni E, Colangelo T, Rizzo F, Magnani E, Muccillo L, Giurato G, Mancini M, Rizzo S, Riccardi M, Sahnane N, Del Vescovo V, Kishore K, Mandruzzato M, Macchi F, Pelizzola M, Denti MA, Furlan D, Weisz A, Colantuoni V, Chiarugi P, Bonapace IM. DNA methylation variations are required for epithelial-to-mesenchymal transition induced by cancer-associated fibroblasts in prostate cancer cells. Oncogene. 2017 Jun 5. doi: 10.1038/onc.2017.159. [Epub ahead of print] PubMed PMID: 28581528.
  • Lo Piccolo L, Attardi A, Bonaccorso R, Li Greci L, Giurato G, Ingrassia AM, Onorati MC. ISWI ATP-dependent remodeling of nucleoplasmic ω-speckles in the brain of Drosophila melanogaster. J Genet Genomics. 2017 Feb 20;44(2):85-94. doi: 10.1016/j.jgg.2016.12.002. Epub 2016 Dec 22. PubMed PMID: 28209301.
  • Panero R, Rinaldi A, Memoli D, Nassa G, Ravo M, Rizzo F, Tarallo R, Milanesi L, Weisz A, Giurato G*. iSmaRT: a toolkit for a comprehensive analysis of small RNA-Seq data. Bioinformatics. 2017 Mar 15;33(6):938-940. doi: 10.1093/bioinformatics/btw734. PubMed PMID: 28057684.*Co-corresponding author.
  • Santangelo L, Giurato G*, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A, Tripodi M. The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Cell Rep. 2016 Oct 11;17(3):799-808. doi: 10.1016/j.celrep.2016.09.031. PubMed PMID: 27732855.*Co-first author.
  • Caporali S, Alvino E, Lacal PM, Levati L, Giurato G, Memoli D, Caprini E, Antonini Cappellini GC, D'Atri S. Targeting the PI3K/AKT/mTOR pathway overcomes the stimulating effect of dabrafenib on the invasive behavior of melanoma cells with acquired resistance to the BRAF inhibitor. Int J Oncol. 2016 Sep;49(3):1164-74. doi: 10.3892/ijo.2016.3594. Epub 2016 Jun 30. PubMed PMID: 27572607.
  • Rizzo F, Rinaldi A, Marchese G, Coviello E, Sellitto A, Cordella A, Giurato G, Nassa G, Ravo M, Tarallo R, Milanesi L, Destro A, Torzilli G, Roncalli M, Di Tommaso L, Weisz A. Specific patterns of PIWI-interacting small noncoding RNA expression in dysplastic liver nodules and hepatocellular carcinoma. Oncotarget. 2016 Aug 23;7(34):54650-54661. doi: 10.18632/oncotarget.10567. PubMed PMID: 27429044; PubMed Central PMCID: PMC5342370.
  • Guacci A, Chetta M, Rizzo F, Marchese G, De Filippo MR, Giurato G, Nassa G, Ravo M, Tarallo R, Rocco T, Operto FF, Weisz A, Coppola G. Phenytoin neurotoxicity in a child carrying new STXBP1 and CYP2C9 gene mutations. Seizure. 2016 Jan;34:26-8. doi: 10.1016/j.seizure.2015.11.004. Epub 2015 Nov 21. PubMed PMID: 26658169.
  • Dago DN, Scafoglio C, Rinaldi A, Memoli D, Giurato G, Nassa G, Ravo M, Rizzo F, Tarallo R, Weisz A. Estrogen receptor beta impacts hormone-induced alternative mRNA splicing in breast cancer cells. BMC Genomics. 2015 May 9;16:367. doi: 10.1186/s12864-015-1541-1. PubMed PMID: 25956916; PubMed Central PMCID: PMC4424892.
  • Cimmino G, Tarallo R, Nassa G, De Filippo MR, Giurato G, Ravo M, Rizzo F, Conte S, Pellegrino G, Cirillo P, Calabro P, Öhman T, Nyman TA, Weisz A, Golino P. Activating stimuli induce platelet microRNA modulation and proteome reorganisation. Thromb Haemost. 2015 Jul;114(1):96-108. doi: 10.1160/TH14-09-0726. Epub 2015 Apr 23. PubMed PMID: 25903651.
  • Ravo M, Cordella A, Rinaldi A, Bruno G, Alexandrova E, Saggese P, Nassa G, Giurato G, Tarallo R, Marchese G, Rizzo F, Stellato C, Biancardi R, Troisi J, Di Spiezio Sardo A, Zullo F, Weisz A, Guida M. Small non-coding RNA deregulation in endometrial carcinogenesis. Oncotarget. 2015 Mar 10;6(7):4677-91. PubMed PMID: 25686835; PubMed Central PMCID: PMC4467107.
  • De Rosa MC, Caputo M, Zirpoli H, Rescigno T, Tarallo R, Giurato G, Weisz A, Torino G, Tecce MF. Identification of Genes Selectively Regulated in Human Hepatoma Cells by Treatment With Dyslipidemic Sera and PUFAs. J Cell Physiol. 2015 Sep;230(9):2059-66. doi: 10.1002/jcp.24932. PubMed PMID: 25639214.
  • Stellato C, Nassa G, Tarallo R,Giurato G, Ravo M, Rizzo F, Marchese G, Alexandrova E, Cordella A, Baumann M, Nyman TA, Weisz A, Ambrosino C. Identification of cytoplasmic proteins interacting with unliganded estrogen receptor α and β in human breast cancer cells. Proteomics. 2015 Jun;15(11):1801-7. doi: 10.1002/pmic.201400404. Epub 2015 Mar 18. PubMed PMID: 25604459.
  • Hashim A, Rizzo F, Marchese G, Ravo M, Tarallo R, Nassa G, Giurato G, Santamaria G, Cordella A, Cantarella C, Weisz A. RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget. 2014 Oct 30;5(20):9901-10. PubMed PMID: 25313140.
  • Rizzo F, Hashim A, Marchese G, Ravo M, Tarallo R, Nassa G, Giurato G, Rinaldi A, Cordella A, Persico M, Sulas P, Perra A, Ledda-Columbano GM, Columbano A, Weisz A. Timed regulation of P-element-induced wimpy testis interacting RNA expression during rat liver regeneration. Hepatology. 2014 Sep;60(3):798-806. doi: 10.1002/hep.27267. Epub 2014 Jul 1. PubMed PMID: 24930433.
  • Nassa G, Tarallo R, Giurato G, De Filippo MR, Ravo M, Rizzo F, Stellato C,Ambrosino C, Baumann M, Lietzèn N, Nyman TA, Weisz A. Post-transcriptionalregulation of human breast cancer cell proteome by unliganded estrogen receptor β via microRNAs. Mol Cell Proteomics. 2014 Apr;13(4):1076-90. doi:10.1074/mcp.M113.030403. Epub 2014 Feb 13. PubMed PMID: 24525454; PubMed Central PMCID: PMC3977185.
  • Casale FP, Giurato G, Nassa G, Armond JW, Oates CJ, Corá D, Gamba A, Mukherjee S, Weisz A, Nicodemi M. Single-cell states in the estrogen response of breast cancer cell lines. PLoS One. 2014 Feb 25;9(2):e88485. doi: 10.1371/journal.pone.0088485. eCollection 2014. PubMed PMID: 24586334; PubMed Central PMCID: PMC3934861
  • Giurato G, De Filippo M.R, Rinaldi A, Hashim A, Nassa G, Ravo M, Rizzo F, Tarallo R, Weisz A. iMir: An Integrated pipeline for high-throughput analysis of small non-coding data obtained by miRNA-Seq. BMC Bioinformatics. 2013 Dec 13;14:362. doi: 10.1186/1471-2105-14-362. PubMed PMID: 24330401; PubMed Central PMCID: PMC3878829.
  • De Filippo M.R, Rizzo F, Marchese G, Giurato G, Nassa G, Ravo M, Tarallo R, Pironti E, Vecchi M, Crichiutti G, Capizzi G, Verrotti A, Weisz A, Coppola G. Lack of pathogenic mutations in six patients with MMPSI. Epilepsy Res. 2014 Feb;108(2):340-4. doi:10.1016/j.eplepsyres.2013.11.007. Epub 2013 Nov 16. PubMed PMID: 24315024.
  • Misaggi R, Di Sanzo M, Cosentino C, Bond HM, Scumaci D, Romeo F, Stellato C, Giurato G, Weisz A, Quaresima B, Barni T, Amato F, Viglietto G, Morrone G, Cuda G, Faniello MC, Costanzo F. Identification of H ferritin-dependent and independent genes in K562 differentiating cells by targeted gene silencing and expression profiling. Gene. 2014 Feb 10;535(2):327-35. doi: 10.1016/j.gene.2013.10.067. Epub 2013 Nov 14. PubMed PMID: 24239552.
  • Caputo M, Zirpoli H, De Rosa MC, Rescigno T, Chiadini F, Scaglione A, Stellato C, Giurato G, Weisz A, Tecce MF, Bisceglia B. Effect of low frequency (LF) electric fields on gene expression of a bone human cell line. Electromagn Biol Med. 2014 Dec;33(4):289-95. doi: 10.3109/15368378.2013.822387. Epub 2013 Aug 26. PubMed PMID: 23977831.
  • Ferraro L, Ravo M, Nassa G, Tarallo R, De Filippo MR, Giurato G, Cirillo F, Stellato C, Silvestro S, Cantarella C, Rizzo F, Cimino D, Friard O, Biglia N, De Bortoli M, Cicatiello L, Nola E, Weisz A. Effects of oestrogen on microRNA expression in hormone-responsive breast cancer cells. Horm Cancer. 2012 Jun;3(3):65-78. doi: 10.1007/s12672-012-0102-1. PubMed PMID: 22274890
  • Paris O, Ferraro L, Grober OM, Ravo M, De Filippo MR, Giurato G, Nassa G, Tarallo R, Cantarella C, Rizzo F, Di Benedetto A, Mottolese M, Benes V, Ambrosino C, Nola E, Weisz A. Direct regulation of microRNA biogenesis and expression by estrogen receptor beta in hormone-responsive breast cancer. Oncogene. 2012 Sep 20;31(38):4196-206. doi: 10.1038/onc.2011.583. Epub 2012 Jan 9. PubMed PMID: 22231442
  • Grober OM, Mutarelli M, Giurato G, Ravo M, Cicatiello L, De Filippo MR, Ferraro L, Nassa G, Papa MF, Paris O, Tarallo R, Luo S, Schroth GP, Benes V, Weisz A. Global analysis of estrogen receptor beta binding to breast cancer cell genome reveals an extensive interplay with estrogen receptor alpha for target gene regulation. BMC Genomics. 2011 Jan 14;12:36. doi: 10.1186/1471-2164-12-36. PubMed PMID: 21235772; PubMed Central PMCID: PMC3025958.