unisa ITA  unisa ENG


LabMedMolGe - Team

Valerio Gigantino

Valerio Gigantino


e-mail: valeriogigantino@gmail.com

 

Laurea in:  Biotecnologie Mediche

 

Dottorato in:  Oncologia molecolare e traslazionale e tecnologie medico chirurgiche innovative (2017 - in corso)


Competenze ed attività di ricerca:  Sviluppo delle principali tecniche di Biologia Molecolare e Genomica come estrazione di Acidi Nucleici (DNA, RNA e small RNA) e proteine da linee cellulari, tessuti e fluidi biologici; PCR quantitativa e qualitativa: Real Time PCR, RT PCR e Multiplex PCR.

 

Pubblicazioni:
  • Gigantino V, Salvati A, Giurato G, Palumbo D, Strianese O, Rizzo F, Tarallo R, Nyman TA, Weisz A, Nassa G. Identification of Antiestrogen-Bound Estrogen Receptor α Interactomes in Hormone-Responsive Human Breast Cancer Cell Nuclei. Proteomics. 2020 Oct;20(19-20):e2000135. doi: 10.1002/pmic.202000135. Epub 2020 Sep 16. PMID: 32865868.
  • Salvati A, Gigantino V, Nassa G, Mirici Cappa V, Ventola GM, Cracas DGC, Mastrocinque R, Rizzo F, Tarallo R, Weisz A, Giurato G. Global View of Candidate Therapeutic Target Genes in Hormone-Responsive Breast Cancer. Int J Mol Sci. 2020 Jun 6;21(11):4068. doi: 10.3390/ijms21114068. PMID: 32517194; PMCID: PMC7312026.
  • Salvati A, Gigantino V, Nassa G, Giurato G, Alexandrova E, Rizzo F, Tarallo R, Weisz A. The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells. Cancers (Basel). 2019 Nov 4;11(11):1720. doi: 10.3390/cancers11111720. PMID: 31689915; PMCID: PMC6895927.
  • Nassa G, Giurato G, Salvati A, Gigantino V, Pecoraro G, Lamberti J, Rizzo F, Nyman TA, Tarallo R, Weisz A. The RNA-mediated estrogen receptor α interactome of hormone-dependent human breast cancer cell nuclei. Sci Data. 2019 Sep 16;6(1):173. doi: 10.1038/s41597-019-0179-2. PubMed PMID: 31527615; PubMed Central PMCID: PMC6746822.
  • Nassa G, Salvati A, Tarallo R, Gigantino V, Alexandrova E, Memoli D, Sellitto A, Rizzo F, Malanga D, Mirante T, Morelli E, Nees M, Åkerfelt M, Kangaspeska S, Nyman TA, Milanesi L, Giurato G, Weisz A. Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells. Sci Adv. 2019 Feb 6;5(2):eaav5590. doi: 10.1126/sciadv.aav5590. eCollection 2019 Feb. PubMed PMID: 30775443; PubMed Central PMCID: PMC6365116
  • Tarallo R, Giurato G, Bruno G, Ravo M, Rizzo F, Salvati A, Ricciardi L, Marchese G, Cordella A, Rocco T, Gigantino V, Pierri B, Cimmino G, Milanesi L, Ambrosino C, Nyman TA, Nassa G, Weisz A. The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading. Genome Biol.2017 Oct 6;18(1):189. doi: 10.1186/s13059-017-1321-0. PubMed PMID: 29017520.
Riassunti pubblicati in atti di convegni nazionali/internazionali:
  • Gigantino V, Salvati A, Nassa G, Giurato G, Rizzo F, Mirici Cappa V, Memoli D, Weisz A, Tarallo R. Functional effect of DOT1L pharmacological inhibi-tion in ERα-positive Ovarian Cancer cells. 61th Annual Meeting of the Can-cer Society. 6-8 November 2019, Naples, Italy.
  • Gigantino V, Salvati A, Alexandrova E, Giurato G, Memoli D, Rizzo F, Sellitto A, Malanga D, Nyman TA, Tarallo R, Weisz A and Nassa G. Pharmacological inhibition of the histone methyltransferase DOT1L as a new epigenetic approach to therapy for endocrine resistant breast cancer. 1st INTERNA-TIONAL AND 32nd Annual Conference of Italian Association of Cell Cultu-res (AICC). 1-2 October 2019, Catanzaro, Italy.
  • Giurato G, Nassa G, Salvati A, Tarallo R, Rizzo F, Gigantino V, Mautone L, Nyman TA, Weisz A. "Analysis of estrogen-mediated signaling reveals a novel epigenetic target for endocrine therapy-resistant breast cancer treatment". Proceeding of 4th National Congress SIPMEL "Pathology and Laboratory Medicine 4.0"; 23-25 October 2018, Catania (Italy).
  • Montalcini Y, Scicchitano S, Santamaria G, Gigantino V, Chiarella E, Aloisio A, Weisz A, Bond H M , Mesuraca M. Zinc Finger Protein 521 influences Notch signalling in ovarian carcinoma cells. Proceeding of 1st and 32nd Annual Conference of Italian Association of Cell Coultures (AICC). 1-2 October, Catanzaro (Italy).
  • Nassa G, Salvati A, Giurato G, Tarallo R, Rizzo F, Gigantino V, Mautone L, Nyman TA, Weisz A. The histone methyl transferase DOT1L is a novel epigenetic target for therapy of endocrine-therapy resistant breast cancer. Proceeding of 30th Pezcoller Foundation; 25-26 June 2018, Trento (Italy).
  • Weisz A, Nassa G, Giurato G, Salvati A, Gigantino V, Nyman TA, Milanesi L, Tarallo R. A proteomics approach for identification of molecular determinants of the oncosuppressive actions of Estrogen Receptor beta in breast cancer. III International Symposium on Profiling-ISPROF 2017 Caparica, Lisbon (Portugal). 4-7 September 2017.